The Detection Of Oxidative Stress

Several parameters are used for the detection of oxidative stress, which measure lipid peroxidation as a measure for oxidative stress in general.

On this behalf, quantification of F2-isoprostanes from biologic fluids and tissues has an important role in the detection and measurement of lipid peroxidation in vivo18. F2-isoprostanes are prostanoids produced independently of cyclooxygenase by free radical-catalyzed peroxidation of arachidonic acid-containing lipids.

Another commonly used marker for oxidative stress is malondialdehyde (MDA)18'19, a highly reactive three carbon dialdehyde produced as a byproduct of polyunsaturated fatty acid peroxidation and arachidonic acid metabolism.

Lipid peroxides are the products of chemical damage done by oxygen free radicals to the polyunsaturated fatty acids of cell membranes, which can be measured with an assay of total thiobarbituric acid-reactive substances (TBARS) in serum using HPLC18,20. The HPLC separation step isolates the TBARS from potential interfering compounds that can give false elevations in a simple colorimetric assay. The results provide a measure of total serum lipid peroxidation, an indicator of whole body free radical activity.

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